london regional innovarium

Transgenic Service

Gene targeting includes ES cell transfection, drug selection, cell culture, aggregation and transfer. Other Services include:

• Embryo cryo-preservation and rederivation by embryo transfer
• Purification of DNA fragments, supplied by clients, for pronuclear injection
• Generation of founder transgenic mice

Gene Targeting Service

• Complete gene targeting experiment, including electroporation of a gene targeted vector into a desired ES cell line, followed by drug selection, freezing of ES cell lines and screening by PCR or Southern Blotting
• Maintenance of an investigators ES cell line in culture for electroporation, morula aggregation or blastocyst microinjection
• Grow and supply investigators with mouse ES cells or primary fibroblast feeder cells for their own gene targeting experiments
• Morula aggregation and transfer to recipient female mice
• CRE- mediated excision

• Embryo cryo-preservation
• Rederivation